Ecotypic Variations Affected the Biological Effectiveness of Thymus daenensis Celak Essential Oil

Table 3

Minimum inhibitory concentration (MIC, mg/ml) and minimum bactericidal concentration (MBC, mg/ml) values for ecotypic T. daenensis essential oils.

Bacteria

Ampicillin

TD1

TD2

TD3

TD4

TD5

TD6

TD7

TD8

TD9

TD10

Escherichia coli (Gram-negative)

MIC

0.002 ± 0.00^a

0.29 ± 0.00^b,c,d

0.39 ± 0.15^c,d

0.15 ± 0.00^a,b

0.15 ± 0.00^a,b

0.21 ± 0.08^a,b,c

0.44 ± 0.17^d

0.1 ± 0.08^a,b

0.14 ± 0.00^a,b

0.28 ± 0.00^b,c,d

0.30 ± 0.00^b,c,d

MBC

0.064 ± 0.00^a

0.58 ± 0.00^b

0.79 ± 0.30^b

0.15 ± 0.00^a

0.3 ± 0.00^a

0.28 ± 0.00^a

0.58 ± 0.00^b

0.55 ± 0.00^b

0.28 ± 0.00^a

0.28 ± 0.00^a

0.59 ± 0.00^b

Bacillus subtilis (Gram-positive)

MIC

<0.0005 ± 0.00^a

0.44 ± 0.17^c,d

0.53 ± 0.00^d

0.46 ± 0.18^c,d

0.3 ± 0.00^b,c

0.14 ± 0.00^a,b

0.29 ± 0.00^b,c

0.14 ± 0.00^a,b

0.14 ± 0.00^a,b

0.28 ± 0.00^b,c

0.30 ± 0.00^b,c,d

MBC

<0.0005 ± 0.00^a

0.62 ± 0.08^d

1.05 ± 0.00^e

0.61 ± 0.00^d

0.3 ± 0.00^c

0.14 ± 0.00^b

0.29 ± 0.00^c

0.14 ± 0.00^b

0.14 ± 0.00^b

0.28 ± 0.00^c

0.59 ± 0.00^d

Staphylococcus aureus MRSA (Gram-positive)

MIC

0.14 ± 0.00^a

0.58 ± 0.00^b,c

1.05 ± 0.00^d

0.86 ± 0.18^c,d

0.45 ± 0.17^a,b

0.42 ± 0.16^a,b

0.44 ± 0.17^a,b

0.41 ± 0.16^a,b

0.42 ± 0.16^a,b

0.56 ± 0.00^b,c

0.30 ± 0.00^a,b

MBC

>0.25 ± 0.00^a

0.84 ± 0.00^c,d

2.14 ± 0.00^e

1.06 ± 0.18^d

0.45 ± 0.17^a,b

0.88 ± 0.00^d

0.44 ± 0.17^a,b

0.41 ± 0.16^a,b

0.56 ± 0.00^b,c

0.56 ± 0.00^b,c

0.30 ± 0.00^a,b

Salmonella typhimurium (Gram-negative)

MIC

0.003 ± 0.001^a

0.15 ± 0.00^b,c

0.53 ± 0.00^e

0.15 ± 0.00^b,c

0.11 ± 0.04^a,b,c

0.07 ± 0.00^a,b

0.07 ± 0.00^a,b

1.09 ± 0.00^f

0.21 ± 0.08^c,d

0.28 ± 0.00^d

0.22 ± 0.09^c,d

MBC

0.016 ± 0.00^a

0.30 ± 0.00^c

1.20 ± 0.00^e

0.28 ± 0.00^c

0.29 ± 0.00^c

0.14 ± 0.00^b

0.14 ± 0.00^b

1.56 ± 0.00^f

0.22 ± 0.09^b,c

0.56 ± 0.00^d

0.30 ± 0.00^c

Enterococcus faecalis (Gram-positive)

MIC

<0.0005 ± 0.00^a

0.15 ± 0.00^b,c

1.05 ± 0.00^f

0.15 ± 0.00^b,c

0.11 ± 0.04^a,b

0.14 ± 0.00^a,b,c

0.15 ± 0.00^b,c

0.55 ± 0.00^e

0.42 ± 0.16^d,e

0.28 ± 0.00^c,d

0.30 ± 0.00^b,c

MBC

0.01 ± 0.00^a

0.58 ± 0.00^d

1.05 ± 0.00^e

0.15 ± 0.00^b

0.15 ± 0.00^b

0.14 ± 0.00^b

0.15 ± 0.00^b

0.55 ± 0.00^d

0.56 ± 0.00^d

0.28 ± 0.00^c

0.30 ± 0.00^c

Staphylococcus aureus (Gram-positive)

MIC

0.08 ± 0.01^a

0.58 ± 0.00^a,b

1.58 ± 0.61^c

0.91 ± 0.35^b,c

0.9 ± 0.35^a,b,c

0.56 ± 0.00^a,b

0.44 ± 0.17^a,b

0.82 ± 0.32^a,b,c

1.12 ± 0.00^b,c

0.84 ± 0.32^a,b,c

0.89 ± 0.34^a,b,c

MBC

0.08 ± 0.05^a

0.58 ± 0.00^a,b

2.11 ± 0.00^b,c

0.91 ± 0.35^a,b,c

0.9 ± 0.35^a,b,c

0.56 ± 0.00^a,b

1.45 ± 1.00^a,b,c

2.73 ± 1.89^c

1.12 ± 0.00^a,b,c

1.12 ± 0.00^a,b,c

2.49 ± 0.00^b,c

Pseudomonas aeruginosa (Gram-negative)

MIC

>0.25

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

MBC

>0.25

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

>5.12

According to the homogeneity of variances using an ANOVA analysis, values in the same row with common letters are not statistically different ().