Gyogamdan, a Traditional Medicine Prescription, Ameliorated Dermal Inflammation and Hyperactive Behavior in an Atopic Dermatitis Mouse Model Exposed to Psychological Stress

Figure 4

Effects of GGDE on inflammatory mediator release in HaCaT and HMC-1 cells. (a) The HaCaT cell viabilities were assessed using XTT assays. (b) Effects of GGDE on TI (TNF-α and IFN-γ)-induced TARC and MDC productions. (c) Western blot of p-STAT1, STAT1, p-IκB-α, IκB-α, β-actin, NF-κB p65, and lamin B in HaCaT cells. (d) HMC-1 cell viabilities were assessed using XTT assays. (e) Effects of GGDE on CRH/SP-induced VEGF production. (f) Western blot of p-ERK, ERK, p-p38, p38, p-JNK, JNK, and β-actin in HMC-1 cells. Dexamethasone (DEX) was used as a positive control. The results are presented as means ± SDs (n = 3 per experiment). , vs. CON; , vs. T/I-treated cells; , vs. CRH/SP-treated cells.

(a)

(b)

(c)

(d)

(e)

(f)