12-Epi-Napelline Inhibits Leukemia Cell Proliferation via the PI3K/AKT Signaling Pathway In Vitro and In Vivo

Figure 2

12-Epi-napelline blocked cell cycle arrest in leukemia cells. K-562 and HL-60 cells were treated with 12-epi-napelline for 24 h and 48 h. To investigate the cell cycle, cells were stained with propidium iodide (PI) according to the manufacturer’s instructions. (a, d) Typical flow cytometric graph of the cell cycle of K-562 and HL-60 cells. (b, c, e, f) The percentage of cells in each cell cycle phase. The results showed that 12-epi-napelline blocked cell cycle arrest in the G0/G1 phase in K-562 and HL-60 cells. (g) The typical graph of cells (×100). Data represented the mean ± standard deviation from three independent experiments (and compared to the control, n = 3).

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