12-Epi-Napelline Inhibits Leukemia Cell Proliferation via the PI3K/AKT Signaling Pathway In Vitro and In Vivo

Figure 4

12-Epi-napelline suppressed the PI3K/AKT/mTOR signaling pathway. K-562 and HL-60 cells were treated with 12-epi-napelline for 24 h. The total protein was extracted as previously described. (a, c) Western blot analyses of PI3K, AKT, p-AKT, and mTOR. The PI3K/AKT/mTOR pathway was suppressed by 12-epi-napelline. (b, d) The graph depicted densitometric analyses of PI3K, AKT, p-AKT, and mTOR normalized by GAPDH (and compared to the control, n = 3).

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