The Herbal Cocktail GSYJ Attenuated Airway Inflammatory Cell Infiltration in a Chronic Asthmatic Mouse Model

Figure 6

Effect of GSYJ on RAW264.7 macrophage cells stimulated with LPS. (a) Cells were incubated with GSYJ for 30 minutes and then stimulated with LPS for 18 hours. GSYJ exerts a dose-dependent inhibitory effect on IL-1β, IL-6, RANTES, and iNOS mRNA expression. (b) Cells were incubated with GSYJ for 30 minutes and then stimulated with LPS for 24 h. Levels of the IL-1β, IL-6, and RANTES proteins were measured using ELISA. (c) Cells were incubated with GSYJ for 30 minutes and then stimulated with LPS for 24 hours and 48 hours prior to iNOS and NO assays, respectively. These profiles are representative of profiles obtained from three independent experiments.

(a)

(b)

(c)