Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling Pathway

<div>ZSD increased Nrf2 activation. (a) The expression of Nrf2 in the cytoplasm and nucleus of the rat ankle joint was evaluated utilizing western blotting, respectively. <i>β</i>-Actin and histone H3 were utilized as internal references, respectively. (b) The Nrf2 mRNA level in the rat ankle joint was detected by qRT-PCR. <i>β</i>-Actin was utilized as an internal control. 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602Z"></path></g><g transform="matrix(.013,0,0,-0.013,61.846,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> compared with the MSU group. (c) The level of Nrf2 in the rat ankle joint was assessed via the immunofluorescence assay. The scale bar = 50 <i>μ</i>m. Arrows represented Nrf2-positive cells. Nrf2: nuclear factor-E2-related factor 2.</div>

Evidence-Based Complementary and Alternative Medicine

fig4

Figure 4

Figure 4: Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling Pathway 

Figure 4 | Zisheng Shenqi Decoction Ameliorates Monosodium Urate-Mediated Gouty Arthritis in Rats via Promotion of Autophagy through the AMPK/mTOR Signaling Pathway 