QiangGuYin Modulates the OPG/RANKL/RANK Pathway by Increasing Secretin Levels during Treatment of Primary Type I Osteoporosis

Figure 4

Effect of QiangGuYin (QGY) treatment on the protein expression of secretin, OPG, RANK, and RANKL in the femur tissue. (a–e) The protein expression of secretin and OPG was decreased, whereas that of RANK and RANKL was increased, after secretin knockdown. The expression of secretin and OPG was increased, whereas that of RANK and RANKL was significantly decreased in the Sec-kno + Q group, as compared to that in the Sec-kno group. Data are expressed as the mean ± SD (n = 3). vs. the Con group; vs. the Sec-kno group. Groups were as follows: control group (Con), secretin overexpression group (Sec-over), secretin overexpression control group (Sec-over-Con), secretin overexpression + QGY group (Sec-over + Q), secretin knockdown group (Sec-kno), secretin knockdown control group (Sec-kno-Con), and secretin knockdown + QGY group (Sec-kno + Q).

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