QiangGuYin Modulates the OPG/RANKL/RANK Pathway by Increasing Secretin Levels during Treatment of Primary Type I Osteoporosis

Figure 5

Effect of QiangGuYin (QGY) treatment on the mRNA expression of OPG, RANK, and RANKL in the femur tissue. (a–c) The mRNA expression of OPG was decreased, whereas that of RANK and RANKL was increased, after secretin knockdown. The mRNA expression of OPG was increased, whereas that of RANK and RANKL was significantly decreased, in the Sec-kno + Q group, compared to levels in the Sec-kno group. Data are expressed as the mean ± SD (n = 6). vs. the Con group; vs. the Sec-kno group. Groups were as follows: control group (Con), secretin overexpression group (Sec-over), secretin overexpression control group (Sec-over-Con), secretin overexpression + QGY group (Sec-over + Q), secretin knockdown group (Sec-kno), secretin knockdown control group (Sec-kno-Con), and secretin knockdown + QGY group (Sec-kno + Q).

(a)

(b)

(c)