Immunological Differences in Human Peripheral Blood Mononuclear Cells Treated with Traditional Japanese Herbal Medicines Hochuekkito, Juzentaihoto, and Ninjin’yoeito from Different Pharmaceutical Companies

Figure 3

Cytokine concentrations in the supernatant of PBMCs treated with 0–400 μg/mL HET, JTT, or NYT manufactured by TJ (black), KR (red), and KO (cyan). After 72 h of culture, the supernatant was analyzed to determine the (a) IL-6, (b) IL-10, (c) IL-17A, (d) IFN-γ, (e) TNF-α, and (f) TGF-β concentrations using bead-array followed by flow cytometry, as described in the Materials and Methods. Data are shown as mean + SD of more than three independent experiments; they were analyzed using two-way ANOVA followed by Dunnett’s multiple comparisons test and Tukey’s multiple comparisons test. vs. control (0 μg/mL) group, and ^† vs. the same concentration group among TJ, KR, and KO. HET, Hochuekkito; JTT, Juzentaihoto; NYT, Ninjin’yoeito; TJ, Tsumura; KR, Kracie; KO, Kotaro.

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