Electroacupuncture Synergistically Inhibits Proinflammatory Cytokine Production and Improves Cognitive Function in Rats with Cognitive Impairment due to Hepatic Encephalopathy through p38MAPK/STAT3 and TLR4/NF-κB Signaling Pathways

Table 1

Sequences of primers used in real-time polymerase chain reactions.

Genes	Sequences of primers	GenBank accession number	Annealing Tm (°C)	Product size (bp)
iNOS	5′CTTGGAGCGAGTTGTGGATTGT3′	NM_012611.3	60	148
	5′GGTAGTGATGTCCAGGAAGTAGGTG 3′
TNF-α	5′CCACCACGCTCTTCTGTCTACTG3′	NM_012675.3	60	151
	5′ TGGGCTACGGGCTTGTCACT 3′
IL-1β	5′CTGTGACTCGTGGGATGATGA3′	NM_031512.2	60	161
	5′ CCACTTGTTGGCTTATGTTCTGTC 3′
IL-6	5′CAGCGATGATGCACTGTCAGA3′	NM_012589.2	60	287
	5′ GGAGAGCATTGGAAGTTGGGG 3′
TLR4	5′AGCCTTGAATCCAGATGAAAC3′	NM_019078	60	135
	5′ ACAGCAGAAACCCAGATGAA 3′
MyD88	5′TCCAACAGAAGCGACTGAT3′	NM_198130	60	83
	5′ GCAGATAGTGATGAACCGTAG 3′
NF-κB	5′GCTCCTTTTCTCAAGCCGATGT3′	NM_199267.2	60	145
	5′ CGTAGGTCCTTTTGCGTTTTTC 3′
P38MAPK	5′TGTGATTGGTCTGTTGGATGTGT3′	NM_031020.2	60	200
	5′ TGTGGATTATGTCAGCCGAGTG3′
STAT3	5′GCCATCCTAAGCACAAAGCC3′ 5′GGGAATGTCAGGGTAGAGGTAGA3′	NM_012747.2	60	249
β-Actin	5′CCTCTATGCCAACACAGT3′	NM_031144	60	155
	5′ AGCCACCAATCCACACAG 3′