Research Article

Icariin and Icariside II Reciprocally Stimulate Osteogenesis and Inhibit Adipogenesis of Multipotential Stromal Cells through ERK Signaling

Figure 3

Quantification of alizarin red S staining during osteogenesis of MSCs. (a–d). Alizarin red S staining (100×): (a) Osteogenic supplements (OS) medium. (b) OS + NaF (1 µM). (c) OS + icariin (1 µM). (d) OS + icariside II (1 µM). (e-f) Quantification of ARS staining: (e) Icariin. (f) Icariside II. and vs. the OS group. Scale bar = 20 µM.