Hypermethylation of DLG3 Promoter Upregulates RAC1 and Activates the PI3K/AKT Signaling Pathway to Promote Breast Cancer Progression

Figure 4

DLG3 can inhibit RAC1 expression in breast cancer cells. (a) qRT-PCR-based assessment of RAC1 expression in paracancerous tissues (normal) and breast cancer (BC) tissues; (b) evaluation of RAC1 protein expression in BC and normal tissues by western blot; (c) determination of RAC1 expression in BC cells and MCF10A by qRT-PCR; (d) qRT-PCR-based measurement of RAC1 expression in BC cells after the overexpression or interference of DLG3; (e) western blot-based assessment of RAC1 protein expression in BC cells after the overexpression or interference of DLG3; after treatment of DNA methylation inhibitor 5-aza-dC, (f) DLG3 methylation level in the cells was measured by bisulfite sequencing PCR; (g) DLG3 and RAC1 expression was measured by qRT-PCR; and (h) western blot was utilized for detecting DLG3 and RAC1 protein expression in MCF cells. ; .

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