Idebenone-Activating Autophagic Degradation of α-Synuclein via Inhibition of AKT-mTOR Pathway in a SH-SY5Y-A53T Model of Parkinson’s Disease: A Network Pharmacological Approach

Figure 6

Effect of Idebenone on α-synuclein degradation and activation of autophagy in the SH-SY5Y-A53T PD cell line. (a) Western blot images of α-synuclein levels after lentiviral transfection; . (b) Histogram of SH-SY5Y-A53T cell viability at different concentrations of Idebenone; ns, not significant, , , and . (c) Western blot images of α-synuclein accumulation, LC3-II, and p62 after Idebenone administration at concentrations ranging from 0 μM to 2.0 μM, and histograms of the relative protein expression of α-synuclein, LC3-II, and p62 in each group vs. “SH-SY5Y-A53T cells without Idebenone treatment,” , , , and . (d) Western blot images of α-synuclein accumulation, LC3-II in each group, and histograms of the relative protein expression of α-synuclein and LC3-II in each group vs. “the control group without any treatment,” . SH-SY5Y-A53T: SH-SY5Y cells overexpressing A53T mutant α-synuclein; SH-SY5Y-CON: SH-SY5Y cells transfected with a negative control plasmid; SH-SY5Y-NOR: SH-SY5Y cells without transfection; IDE: Idebenone.

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