Bioinformatics and Network Pharmacology-Based Approaches to Explore the Potential Mechanism of the Antidepressant Effect of Cyperi Rhizoma through Soothing the Liver

Figure 4

Identification of potential targets of CR in combating MDD through modulating the liver response to LPS and glucose metabolism. (a) Normalization of 3 control samples and 3 MDD samples. (b) Principal component analysis (PCA) and uniform manifold approximation and projection (UMAP) before DEG analysis. PCA and UMAP were applied to 3 MDD liver tissues (red) and 3 CTL liver tissues (blue) characterized by the gene expression of all probes on Affymetrix Rat 230 2.0 Array. (c) Volcano diagrams of MDD-related DEGs. Red dots represent upregulated DEGs, and green dots represent downregulated DEGs. (d) Heatmaps of MDD-related DEGs. The colour shift from blue to red indicates a trend from low to high expression, respectively. (e) Venn diagram of DEGs, LPS-related genes, and diabetes-related genes. (f) Relative expression levels of PAI-1 and IL-1B in the MDD group compared with the control group. vs. control.

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