Effects of Various Preextraction Treatments of Crinum asiaticum Leaf on Its Anti-Inflammatory Activity and Chemical Properties

Figure 2

HPLC chromatograms of blank (DMSO) (a), standard lycorine solution (0.5 mg/mL) (b), CAA extract at 10 mg/mL (c), CAM extract at 10 mg/mL (d), CAG extract at 10 mg/mL, (e) and CAC extract at 10 mg/mL (f) by using HPLC analysis with mobile phase consisting of 0.1%v/v triethylamine in water adjusting pH to 3.0 with phosphoric acid (A) and acetonitrile (B). Gradient elution was programmed as follows: 0–5 min, 5%B; 25 min, 30%B; 25.1–30 min, 80%B; 30.1–35 min, 5%B and detected at the wavelength of 290 nm. The chromatograms of standard lycorine solution (b) and crude extracts solutions (c), (d), (e), and (f) show similar peaks at RT = 5.8.

(a)

(b)

(c)

(d)

(e)

(f)