Effects of Various Preextraction Treatments of Crinum asiaticum Leaf on Its Anti-Inflammatory Activity and Chemical Properties
Table 2
Inhibitory effect of the ethanolic extracts from different preextraction treatments of C. asiaticum on LPS-induced NO production from RAW 264.7 cells (mean ± SEM, n = 3).
Sample
% inhibition of NO production from RAW 264.7 cells at various concentrations (µg/mL) (% cytotoxicity)
IC50 ± SEM (µg/mL)
0.1
0.2
0.4
0.8
1
10
30
50
100
CAA
—
—
—
—
-2.58 ± 2.36
23.49 ± 5.96
96.48 ± 1.82
97.49 ± 1.23
98.26 ± 0.50
16.66 ± 1.42
(−6.08 ± 12.47)
(−10.10 ± 9.18)
(2.95 ± 0.82)
(−15.27 ± 9.52)
(23.91 ± 0.85)
CAM
—
—
—-
—
−10.80 ± 8.07
11.03 ± 2.75
83.44 ± 4.58
102.39 ± 5.33
96.12 ± 0.56
20.42 ± 0.57
(−0.63 ± 8.29)
(−3.40 ± 2.59)
(8.07 ± 1.05)
(9.45 ± 0.52)
(12.00 ± 5.64)
CAG
—
—
—
—
−4.63 ± 1.18
6.78 ± 2.44
83.37 ± 1.62
96.09 ± 0.37
101.55 ± 3.49
21.35 ± 0.56
(−1.94 ± 6.54)
(2.62 ± 6.01)
(−5.60 ± 12.94
(3.72 ± 7.17)
(10.41 ± 8.68)
CAC
—
—
—
—
−6.33 ± 1.28
−1.25 ± 7.92
81.21 ± 2.68
96.61 ± 2.71
96.59 ± 0.99
22.48 ± 1.09
(2.33 ± 6.41)
(0.92 ± 2.94)
(7.23 ± 2.52)
(11.66 ± 5.08)
(17.07 ± 4.07)
Prednisolone
32.85 ± 7.64
—
—
—
48.04 ± 2.07
64.11 ± 7.46
—
79.16 ± 3.18
—
1.05 ± 0.13
(2.02 ± 3.15)
(5.35 ± 3.43)
(3.88 ± 4.68)
(9.64 ± 4.74)
Diclofenac
—
—
—
—
—
—
—
−8.57 ± 0.86
1.16 ± 0.90
>100
(−20.36 ± 0.68)
(11.88 ± 1.49)
Lycorine
−10.93 ± 2.34
7.46 ± 0.44
50.72 ± 0.37
82.63 ± 3.90
86.86 ± 3.66(t)
—
—
—
—
0.40 ± 0.00
(5.46 ± 4.37)
(9.98 ± 8.20)
(15.29 ± 4.92)
(27.35 ± 1.30)
(36.96 ± 4.15)
(—) means not tested. (t)cytotoxicity greater than 30%.
