Derrone Inhibits Platelet Aggregation, Granule Secretion, Thromboxane A₂ Generation, and Clot Retraction: An In Vitro Study

Figure 5

Inhibitory action of derrone on fibrinogen binding to αIIb/β3 and fibronectin adhesion. (a) Flow cytometry histograms for fibrinogen binding. (a) Intact platelets (base); (b) collagen (2.5 μg/mL); (c) collagen (2.5 µg/mL) + derrone (15 μM); (d) collagen (2.5 μg/mL) + derrone (30 μM); (e) collagen (2.5 μg/mL) + derrone (45 μM); (f) collagen (2.5 μg/mL) + derrone (60 μM). (b) Inhibitory action of derrone on collagen-induced fibrinogen binding (%). (c) Effects of derrone on collagen-induced fibronectin adhesion. Measurement of fibrinogen binding and fibronectin adhesion was carried out as described in the Materials and Methods section. Data are expressed as mean ± standard deviation (n = 4). and versus the collagen-stimulated human platelets.

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