The RIG-I Signal Pathway Mediated Panax notoginseng Saponin Anti-Inflammatory Effect in Ischemia Stroke

Figure 2

The effect of PNS on RIG signaling pathway and downstream inflammatory factors in OGD-induced BMECs. The OGD-injured BMECs were administrated with 22 μg/ml PNS. siRNA was used to silence RIG-I gene as control. (a) The mRNA levels of RIG-I, Traf2, and NF-κB (p65) were detected by RQ-PCR. (b) The expressions of RIG-I, Traf2, and NF-κB (p65) were detected by western blotting. Quantitative analysis of RIG-I (c), Traf2 (d), and NF-κB (p65) (e) protein levels are shown. (f) The result of p65 DNA binding assay. The content of TNF-α (g) and IL-8 (h) was detected by ELISA. (i) Analysis of the correlation showed that production of TNF-α, IL-8, and RIG-I signaling pathway was significantly correlated. Data are presented as mean ± s.e.m. ^### < 0.001 versus normal group,  < 0.01,  < 0.001 versus model group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)