Synergistic Herb-Herb Interaction of the Antinociceptive and Anti-Inflammatory Effects of Syzygium aromaticum and Rosmarinus officinalis Combination

Figure 5

Isobolographic analysis of the antinociceptive (a) and anti-inflammatory (b) effect of S aromaticum and R. officinalis in combination (1 : 1 ratio). Data represented as mean ± SEM of six rats evaluated in the formalin or carrageenan test in rats. The continuous line represents additivity for all the additive theoretical combinations. The point (○) in the line represents the theoretical additive point, which was calculated from the individual drug EC₅₀ values, in this case, was named (Z_add). The point (●) is the experimental EC₅₀ (Z_mix) indicating significant synergism. Statistical difference using Student’s t-test Z_add versus Z_mix.

(a)

(b)