Antidiarrheal Effect of 80% Methanol Extract and Fractions of Clerodendrum myricoides (Hochst.) Vatke (Lamiaceae) Leaf in Swiss Albino Mice

Table 1

The effects of 80% methanol extract and solvent fractions of the leaf of C. myricoides on the castor oil-induced diarrheal model in mice.

Dose (mg/kg)	Onset of diarrhea	Number of wet feces	Total number of feces	Weight of fresh feces	% diarrhea inhibition
Control	53.43 ± 5.79	9.17 ± 1.17	11.50 ± 1.05	0.38 ± 0.04	—
MeOH 100	73.60 ± 6.97^c2b3	4.83 ± 0.31^a1b3c3	6.17 ± 0.31^a1b2c3	0.22 ± 0.04^a2c1	47.33
MeOH 200	98.27 ± 3.79^a2b2c2	4.17 ± 0.62^a3b2c2	5.33 ± 0.56^a3	0.16 ± 0.02^a3	54.53
MeOH 400	121.93 ± 2.17^a3	2.83 ± 0.31^a3	4.67 ± 0.42^a3	0.13 ± 0.01^a3	69.14
Lop 3	134.77 ± 3.80^a3	2.50 ± 0.22^a3	4.00 ± 0.40^a3	0.10 ± 0.02^a3	72.74
HF 100	57.43 ± 5.31^b2c3	7.83 ± 0.48^b2c3	10.67 ± 1.02^b3b3	0.33 ± 0.02^b3c3	14.61
HF 200	63.27 ± 0.61^b2c3	7.17 ± 0.60^b3c3	9.83 ± 0.75^b3c3	0.29 ± 0.02^b2c3	21.81
HF 400	86.10 ± 7.9^a2b2c3	5.00 ± 0.32^a1b2c2	7.33 ± 0.71^a1b2c2	0.23 ± 0.02^a1b2c2	45.47
CF 100	67.77 ± 5.32^b2c2	5.17 ± 0.31^a1b2c2	7.83 ± 0.33^a1b2c3	0.25 ± 0.04^a1c1	43.62
CF 200	94.60 ± 5.91^a2b2	4.50 ± 0.56^a2	6.33 ± 0.48^a1	0.19 ± 0.02^a3	50.92
CF 400	119.43 ± 4.32^a3	3.17 ± 0.31^a3	4.50 ± 0.72^a3	0.15 ± 0.01^a3	65.43
Control	47.64 ± 4.66	9.83 ± 0.31	11.83 ± 0.33	0.37 ± 0.05	—
AF 100	59.48 ± 2.89^b2c3	6.83 ± 0.48^b2c3	8.67 ± 0.33^b2c3	0.28 ± 0.03^b2c2	30.52
AF 200	82.48 ± 4.98^a2b2c2	4.67 ± 0.49^a2	6.50 ± 0.67^a2c3	0.22 ± 0.02^a2	52.49
AF 400	107.81 ± 4.37^a3	3.67 ± 0.33^a3	5.00 ± 0.37^a3	0.18 ± 0.02^a3	62.67
Lop 3	128.17 ± 3.80^a3	2.67 ± 0.22^a3	4.00 ± 0.40^a3	0.12 ± 0.02^a3	72.84

Values are expressed as mean ± standard error of mean (n = 6), ^acompared to the negative control, ^bcompared to the 400 mg/kg MeOH extract, and ^ccompared to the positive control. MeOH: 80% methanol extract; Lop 3: 3 mg/kg loperamide; CF: chloroform fraction; HF: hexane fraction; AF: aqueous fraction. Controls were given 10 ml/kg distilled water (for aqueous fraction) and 10 mk/kg of 2% Tween 80 (for chloroform fraction, hexane fraction, and crude extract). ¹, ², and ³.