Antidiarrheal Effect of 80% Methanol Extract and Fractions of Clerodendrum myricoides (Hochst.) Vatke (Lamiaceae) Leaf in Swiss Albino Mice

Table 2

The effects of 80% methanol extract and solvent fractions of the leaf of C. myricoides on the castor oil-induced enteropooling model in mice.

Dose (mg/kg)	VSIC (ml)	% of volume inhibition	WSIC (g)	% of weight inhibition
Control	0.75 ± 0.03	—	0.84 ± 0.03	—
MeOH 100	0.50 ± 0.02^a2b1c3	33.33	0.54 ± 0.03^a2b1c3	35.71
MeOH 200	0.42 ± 0.03^a3	44.00	0.46 ± 0.05^a3	45.24
MeOH 400	0.35 ± 0.03^a3	53.33	0.37 ± 0.03^a3	55.95
Lop 3	0.30 ± 0.03^a3	60.00	0.33 ± 0.03^a3	60.71
HF 100	0.68 ± 0.03^b3c3	9.33	0.72 ± 0.03^b3c3	14.29
HF 200	0.60 ± 0.05^b2c3	20.00	0.68 ± 0.05^b2c3	19.05
HF 400	0.51 ± 0.03^a1b2c3	32.00	0.57 ± 0.04^a1b1c2	32.14
CF 100	0.52 ± 0.07^a1b1c3	30.67	0.55 ± 0.05^a1b3c1	34.52
CF 200	0.45 ± 0.04^a3	40.00	0.47 ± 0.03^a3	44.05
CF 400	0.34 ± 0.02^a3	54.69	0.39 ± 0.06^a3	53.57
Control	0.76 ± 0.02	—	0 .87 ± .04	—
AF 100	0.55 ± 0.03^b2c3	27.63	0.64 ± 0.03^b3c3	26.44
AF 200	0.47 ± 0.02^a2b2	38.16	0.52 ± 0.03^a2b1c1	40.23
AF 400	0.42 ± 0.04^a3	44.74	0 .44 ± 0.01^a3	49.43
Lop 3	0.33 ± 0.03^a3	56.58	0.35 ± 0.02^a3	59.77

Values are expressed as mean ± standard error of mean (n = 6), ^acompared with the negative control, ^bcompared with the 400 mg/kg MeOH extract, and ^ccompared with the positive control. VSIC (ml): volume of the small intestinal content; WSIC (g): weight of the small intestinal content; MeOH: 80% methanol extract; Lop: loperamide; CF: chloroform fraction; HF: hexane fraction; AF: aqueous fraction. Controls were given 10 ml/kg 2% Tween 80 (for 80% MeOH extract, chloroform, and hexane fractions) and 10 ml/kg distilled water (for aqueous fraction). ¹, ², and ³.