Antidiarrheal Effect of 80% Methanol Extract and Fractions of Clerodendrum myricoides (Hochst.) Vatke (Lamiaceae) Leaf in Swiss Albino Mice

Table 3

The effects of 80% MeOH extract and solvent fractions of the leaf of C. myricoides on castor oil-induced gastrointestinal motility in mice.

Treatment (mg/kg)	LSI (cm)	DTCM (cm)	PI	% inhibition
Control	52.54 ± 1.54	36.69 ± 2.39	69.83 ± 4.24	—
MeOH 100	56.93 ± 1.34	22.83 ± 1.78^a2b1c3	40.10 ± 3.21^a2b1c1	42.57
MeOH 200	55.50 ± 1.91	17.33 ± 3.57^a3	31.23 ± 2.29^a3	55.29
MeOH 400	54.33 ± 1.23	13.83 ± 0.91^a3	25.46 ± 1.83^a3	63.55
Atr 1	53.25 ± 0.96	12.17 ± 0.91^a3	22.85 ± 1.76^a3	67.27
HF 100	56.75 ± 1.14	33.00 ± 2.59^b3c	58.15 ± 3.90^b3c3	16.73
HF 200	58.17 ± 1.85	30.58 ± 2.89^b3c3	52.57 ± 5.89^b3c3	24.72
HF 400	56.08 ± 0.93	26.25 ± 2.29^a1b2c3	46.81 ± 3.77^a1b2c2	32.97
CF 100	54.17 ± 2.41	23.12 ± 1.74^a2b1c3	42.68 ± 3.22^a2b1c3	38.88
CF 200	57.17 ± 1.62	18.67 ± 1.26^a3	32.66 ± 2.68^a3	53.24
CF 400	53.25 ± 0.91	14.92 ± 1.51^a3	28.02 ± 2.96^a3	59.88
Control^2	55.58 ± 0.76	40.08 ± 1.58	72.11 ± 3.67	—
AF 100	55.24 ± 1.82	28.8 ± 1.45^b2c3	52.14 ± 1.55^b2c3	27.70
AF 200	53.50 ± 0.96	21.17 ± 0.87^a2c2	39.57 ± 2.23^a2c2	45.13
AF 400	55.50 ± 1.55	19.98 ± 1.37^a3	36.00 ± 1.92^a3	50.08
Atr 1	54.83 ± 1.19	13.90 ± 0.99^a3	25.35 ± 2.03^a3	64.84

Values are expressed as mean ± standard error of mean (n = 6), ^acompared to the negative control, ^bcompared to the 400 mg/kg MeOH extract, and ^ccompared to the positive control. LSI (cm): length of the small intestine; DTCM (cm): distance travelled by charcoal meal; PI: peristalsis index; MeOH: 80% methanol extract; Atr: atropine; HF: hexane fraction; CF: chloroform fraction; AF: aqueous fraction. Controls were given 10 ml/kg distilled water (for aqueous fraction) and 10 ml/kg 2% Tween 80 (for 80% MeOH extract, chloroform, and hexane fractions). ¹, ², and ³.