Role of Episamarcandin in Promoting the Apoptosis of Human Colon Cancer HCT116 Cells through the PI3K-Akt Signaling Pathway

Table 3

Primer sequence for fluorescence real-time quantification PCR.

Gene ID	Primer	Sequence (5′to3′)	Length (bp)
NM_001101	β-Actin_F	ATGATGATATCGCCGCGCTC	211
	β-Actin_R	TCGATGGGGTACTTCAGGG
NM_201284	EGFR-F	AGGCACGAGTAACAAGCTCAC	177
	EGFR-R	ATGAGGACATAACCAGCCACC
NM_000314	PTEN-F	TTTGAAGACCATAACCCACCAC	134
	PTEN-R	ATTACACCAGTTCGTCCCTTTC
NM_001242466	PI3Kp85-F	ACCACTACCGGAATGAATCTCT	207
	PI3Kp85-R	GGGATGTGCGGGTATATTCTTC
NM_005163	Akt1-F	GTCATCGAACGCACCTTCCAT	218
	Akt1-R	AGCTTCAGGTACTCAAACTCGT
NM_004958	mTOR-F	GCAGATTTGCCAACTATCTTCGG	114
	mTOR-R	CAGCGGTAAAAGTGTCCCCTG
NM_001322240	Bcl-xl-F	GACTGAATCGGAGATGGAGACC	179
	Bcl-xl-R	GCAGTTCAAACTCGTCGCCT
NM_001126118	p53-F	CAGCACATGACGGAGGTTGT	125
	p53-R	TCATCCAAATACTCCACACGC
NM_000633.3	Bcl-2-F	GGATAACGGAGGCTGGGATG	103
	Bcl-2-R	GGCCAAACTGAGCAGAGTCT
NM_001291428.2	Bax-F	CATGGGCTGGACATTGGACT	137
	Bax-R	AAAGTAGGAGAGGAGGCCGT
NM_001350234.2	PI3Kp110δ-F	TGGACTGCCCCATGGAATTC	203
	PI3Kp110δ-R	GCAGGTGAACACATAGGCCT