Research Article
PAMK Relieves LPS-Induced Enteritis and Improves Intestinal Flora Disorder in Goslings
Table 2
The primers and amplification parameters of target genes.
| | Gene | Primer sequence (5′ to 3′) |
| | TLR4 | F: TTGTGTGCTGAAGGTCCAAG | R: TCCTCAGTTTCCTGGGTCTG | | Occludin | F: TCTTCCACATCAAGCGCATG | R: GGTCGCTTTGGATGTTGGTT | | ZO-1 | F: CAAAGGTGAAGTGTTCCGGG | R: CTCCTCCTGCTGTCTTTGGA | | IL-1β | F: ACGGTGTGGGGACATTCATC | R: AGGCGAAGCTTCTTCTGTGG | | IL-2 | F: TCATCTCGAGCTCTACACACCAA | R: TGCATTCACTTCCGGTGTGAT | | IL-4 | F: GGCATCTACCTCAACTTGCT | R: CTCTTTCGCTACTCGTTGGA | | IL-6 | F: ACGATAAGGCAGATGGTGAT | R: TCCAGGTCTTATCCGACTTC | | IL-10 | F: ATCATGACATGGACCCGGTA | R: ATTGCTCCATGACAGTTGCT | | IFN-γ | F: CCAGATTGTTTCCCTGTACTTG | R: CATCAGAAAGGGTGTCTCTCA | | TGF-β | F: CATCACAGAGACAGGAACCTT | R: CTTTCACATCACCACTGGAA | | IgA | F: GTCACCGTCACCTGGACTACA | R: ACCGATGGTCTCCTTCACATC | | IgG | F: ATCACGTCAAGGGATGCCCG | R: ACCAGGCACCTCAGTTTGG | | IgM | F: GCATCAGCGTCACCGAAAGC | R: TCCGCACTCCATCCTCTTGC | | β-Actin | F: GCACCCAGCACGATGAAAAT | R: GACAATGGAGGGTCCGGATT |
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