Synergistic Regulation of Microglia Gene Expression by Natural Molecules in Herbal Medicine

Figure 1

The plant origin and chemical structure of alantolactone (Ala) and dehydrodiisoeugenol (Deh) and the cytotoxicity assessment of Ala and Deh. Cell viability was performed on 1 × 10⁴ BV2 (mouse microglial cell lines) cells/well in 100 μL of DMEM medium supplemented with 10% FBS and 1% penicillin-streptomycin. All of the alantolactone (Ala) and dehydrodiisoeugenol (Deh) were dissolved in DMSO, and all stock solutions were of 100.0 mM and 1.0 mM, respectively. Cells were treated with different concentrations of Ala and Deh. MTS assay showing the cell viability of BV2 cells treated with different concentrations of Ala and Deh. Results were expressed as mean ± S.D (n = 3). (a, b) Ala; (c, d) Deh. ^∗∗∗∗P <0.0001 indicates statistical significance.

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