Synergistic Regulation of Microglia Gene Expression by Natural Molecules in Herbal Medicine

Figure 6

Validation of RNA-seq results using RT-qPCR. BV2 cells were pretreated with different concentrations of Ala, Deh, and Mix for 12 h then treated with LPS (100 ng/mL) for 6 h. (a) The FKPM value of Csf3, Ccl7, Ccl2, Il1rn, Il1β, Nos2, Tnfα, and Cxcl10 in RNA-seq. (b) The expression level of Csf3, Ccl7, Ccl2, Il1rn, Il1β, Nos2, Tnfα, and Cxcl10 in RT-qPCR. mRNA levels are expressed as percent of nontreated control (NT). Each value represents the mean ± standard deviation of duplicate cultures. ^∗∗∗∗P <0.0001, ^∗∗∗P <0.001, ^∗∗P <0.01, and ns >0.05 indicate statistical significance.

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