Cornel Iridoid Glycoside Ameliorated Alzheimer’s Disease-Like Pathologies and Necroptosis through RIPK1/MLKL Pathway in Young and Aged SAMP8 Mice

Figure 6

Effects of CIG on the RIPK1/MLKL pathway in the cerebral cortex of young and aged SAMP8 mice (western blotting). (a, b) Representative images and quantitative analysis of the expression of RIPK1, phosphorylated MLKL, and total MLKL in the cerebral cortex of mice at 6 months of age and (c, d) at 14 months of age. GAPDH served as an internal loading control, and the relative intensity in the SAMR1 control group was set as 100%. Data are expressed as the mean ± S.E.M., n = 4 each group. and : SAMP8 model group vs. SAMR1 control group; and : drug-treated SAMP8 groups vs. SAMP8 model group. RIPK1, receptor-interacting protein kinase-1; MLKL, mixed lineage kinase domain-like protein; p-MLKL, phosphorylated MLKL at Ser345.

(a)

(b)

(c)

(d)