Effects of a Chlorogenic Acid-Containing Herbal Medicine (LAS^NB) on Colon Cancer

Figure 2

(a) MTT assay. 24 h after exposing CT26, HCT15, HCT116, or MCN460 cells to LAS^NB. LAS^NB inhibited cell proliferation in a concentration-dependent manner and had limited effects on NCM460 cells. (b) Changes in the morphology of CT26 and HCT116 cells after 24 h LAS^NB treatment. (c) Crystal violet assay: CT26, HCT15, and HCT116 cells were treated with LAS^NB, and cell viability was inhibited in a concentration-dependent manner. (d) FCM assay. LAS^NB promoted cell apoptosis after 24 h LAS^NB treatment (dose = 2.979 mg/ml, 3.145 mg/ml, and 2.455 mg/ml for CT26, HCT15, and HCT116 cells, respectively). (e) Scratch width in the different groups; CT26 (E1) and HCT116 (E2) groups were observed at 0, 24, 48 h, and 72 h, respectively. Magnification, 40x; ^∗, ^∗∗, and ^∗∗∗.

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