Optimization of the Purifying Process for Columbianetin-β-D-Glucopyranoside from Angelicae Pubescentis Radix and Evaluation of Its Analgesic Activity Using Hot Plate Test

Table 6

Comparison of the MARs method with other methods in the purification of coumarins in TCMs.

No.	Purification compounds	Source	Type of solvent	Method	Reference
1	Xanthotoxin, isopimpinellin, bergapten, imperatorin, and osthole	Cnidium monnieri (L.) Cusson	Light petroleum–ethyl acetate–methanol–water	HSCCC^a	[23]
2	Xanthotoxin, isopimpinellin, bergapten, imperatorin, and osthole	Cnidium monnieri (L.) Cusson	n-Hexane–ethyl acetate–ethanol–water	HSCCC^a	[24]
3	Xanthotoxin, bergapten, columbianetin acetate, osthole, isoimperatorin, and columbianadin	Angelicae Pubescentis Radix	n-Hexane–ethyl acetate–methanol–water	CCC-PHPLC^b	[25]
4	Columbianetin-β-D-glucopyranoside	Angelicae Pubescentis Radix	Ethanol–water	MARs^c	This work

^aHigh-speed countercurrent chromatography. ^bCountercurrent chromatography and preparative liquid chromatography. ^cMacroporous resins.