[Retracted] Wild Bitter Melon Extract Abrogates Hypoxia-Induced Cell Death via the Regulation of Ferroptosis, ER Stress, and Apoptosis in Microglial BV2 Cells

Figure 1

Hypoxia induces ferroptosis via the Gpx4/SLC7A11 downregulation, decreases cell survival, and induces ER stress via CHOP/Bip upregulation. (a) Changes in lipid ROS levels of control (without 2 μM C11-BODIPY staining), normoxia, and hypoxia cells (with 2 μM C11-BODIPY staining). (b) Mean fluorescence intensity is used to measure lipid ROS levels. (c) WST-1 measures the viability of cells in the indicated groups. (d) The upper panel indicates expression changes of Gpx4 and SLC7A11 proteins. The lower panel indicates the quantitative results of assessing specific proteins using ImageJ. (e) The upper panel indicates the expression changes of CHOP and Bip proteins. The lower panel indicates the quantitative results of assessing specific proteins using Image =J. Three independent replicates were used to calculate the standard deviation (n = 3). . N, normoxia; H, hypoxia.

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