MicroRNA-34a Promotes Ischemia-Induced Cardiomyocytes Apoptosis through Targeting Notch1

Figure 1

MI upregulated miR-34a expression and promoted apoptosis. MI: myocardial infarction group (myocardial infarction model); BC: blank control group (normal); NC: negative control group (sham operation). (a) qRT-PCR is showing the expression of miR-34a in the myocardium of rats. (b) Expression of caspase-3, Bcl-2, Bax, and Notch1 in different groups detected by performing the western blotting assay. (c) The quantified results of the western blotting assay. (d) The results of TUNEL staining. MI: it is area of myocardial infarction, and there are a large number of apoptotic cardiomyocytes with different shades of brown cell nucleus (as indicated by the arrow). BC and NC: they are noninfarct area, and the cardiomyocytes without brown cell nucleus are arranged orderly. (e) The results of HE staining. MI shows few blood vessels and rarely viable myocardium with irregular cell structure and sequence in the infarct area (as indicated by the arrow). BC and NC show that normal cardiomyocytes in the noninfarct area are orderly ( < 0.01 vs NC).

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