Ginsenoside Rg1 Reduces Oxidative Stress Via Nrf2 Activation to Regulate Age-Related Mesenchymal Stem Cells Fate Switch Between Osteoblasts and Adipocytes

Figure 1

Mice treated with Rg1 reduced oxidative stress and intraosseous adipogenesis. Note: (a) A 15‐month‐old mice was used as a mice model. The treatment group was intraperitoneally injected with Rg1 at the dosage of 40 mg/kg every day for 3 months. (b) SOD and MDA levels were measured (n = 5). (c) Hematoxylin-Eosin staining and the number and area of adipocytes (d) in the distal femora. (e-f) Levels of Nrf2, HO-1, and NQO1 by western blot (n = 3). (g) Expression of Ppar-γ and Fabp4 by qRT‐PCR (n = 5). (h) Expression of Runx2 and Osx by qRT‐PCR (n = 5). Scale bar represent 200 μm (c), Data represented by means ± SD in (b) d, (f) (g), (h). (, Student’s t-test).

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