Ginsenoside Rg1 Reduces Oxidative Stress Via Nrf2 Activation to Regulate Age-Related Mesenchymal Stem Cells Fate Switch Between Osteoblasts and Adipocytes

Figure 3

Compared to BMMSCs from young people, BMMSCs from aging people had reduced antioxidant capacity, osteogenic differentiation potential and Nrf2 expression level. Note: (a) Result of immunofluorescence showed the level of Nrf2 in the nucleus in young and elder groups. (b) Nrf2 protein expression in BMMSCs cells was measured by western blot (n = 3). (c) SOD enzyme levels and oxidative stress products MDA, ROS levels were compared between the elder and younger groups (n = 5). (d) Expression of Nrf2, HO-1, and NQO1 by qRT-PCR. (e) Alizarin red staining and quantification (n = 5). (f) ALP staining and quantification (n = 5). (g) Oil red O staining and quantification (n = 5). (h) Expression levels of Runx-2 and Osx by qRT-PCR (n = 5). (i) Expression of Fabp4 and Ppar-γ (n = 5). Scale bar represents 50 μm (a), 100 μm (e-g), Data represented by means ± SD in (b-i). (; Student’s t-test).

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