Ginsenoside Rg1 Reduces Oxidative Stress Via Nrf2 Activation to Regulate Age-Related Mesenchymal Stem Cells Fate Switch Between Osteoblasts and Adipocytes

Figure 6

Rg1 regulates the differentiation of BMMSCs by activating Nrf2 signaling. BMMSCs were treated with ODM for 72 h. The Rg1 group was treated with 20 μg/mL Rg1 for 72 h. The inhibitor group was treated with 20 μg/mL Rg1 and 80 nmol/mL brusatol for 72 h. Note: (a) Immunofluorescence showing that Nrf2 expression occurs in the nucleus. (b-c) Relative expression of Nrf2 and p-Nrf2 by western blot analysis (n = 3), β-actin and Nrf2 was reference. (d-e) Relative expression levels of NQO1 and HO-1 by western blot. (n = 3), β-actin was the reference. Scale bar represents 50 μm (a), Data represented by means ± SD in (d-e). (; one-way ANOVA).

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