Repeated Yueju, But Not Fluoxetine, Induced Sustained Antidepressant Activity in a Mouse Model of Chronic Learned Helplessness: Involvement of CaMKII Signaling in the Hippocampus

Figure 3

Western blots of neural plasticity related signaling in the hippocampus. Control animals (CTL) received vehicle treatment and no training, and animals exposed to chronic learned helplessness (cLH) for 21 days received administration of vehicle (Veh), Yueju pills (YJ), or fluoxetine (Flx). (a) Protein expression levels of NR1 (F (3, 21) = 3.985 for day 7; F (3, 20) = 8.190 for day 26, ). (b) PKA (F (3, 22) = 3.526 for day 7; F (3, 20) = 3.186 for day 26, ), (c) P-CREB/CREB (F(3, 24) = 5.60 for day 7, ; F (3, 19) = 1.182 for day 26, ), (d) P-CaMKII/CaMKII (F (3, 21) = 6.565 for day 7; F (3, 18) = 8.607 for day 26, ), (e) PSD95F (3, 20) = 2.770 for day 7; F (3, 20) = 3.080 for day 26, ), and (f) BDNF (F (3, 20) = 0.5380 for day 7; F (3, 20) = 0.3171 for day 26, ) in the hippocampus. Data are expressed as mean ± S.E.M. , compared to CTL; , compared to Veh (one-way ANOVA and Tukey’s post hoc test). n = 6–8/group.

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