Repeated Yueju, But Not Fluoxetine, Induced Sustained Antidepressant Activity in a Mouse Model of Chronic Learned Helplessness: Involvement of CaMKII Signaling in the Hippocampus

Figure 4

Intracerebroventricular microinjection of KN-62 blocked Yueju induced antidepressant effect. Mice received intracerebroventricular injection of vehicle or KN-62 (5 μmol/site) 30 min prior to Yueju or fluoxetine. Four groups of mice were used to investigate the involvement of CaMKII signaling in the antidepressant-like effect of Yueju or fluoxetine: (1) vehicle plus Sham, (2) vehicle plus KN-62, (3) Yueju or fluoxetine plus Sham, and (4) Yueju or fluoxetine plus KN-62. (a) Tail suspension test (TST) was performed 1 h after the administration of Yueju. (b) Forced swimming test (FST) was performed 3 h after the administration of Yueju. (c) TST was performed 1 h after the administration of fluoxetine. (d) FST was performed 3 h after the administration of fluoxetine. compared with vehicle + Sham group; compared with Yueju + Sham group (two-way ANOVA and Tukey’s post hoc test).

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