Neuroprotective Effects of Curcumin against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury in Cultured Primary Rat Astrocyte by Improving Mitochondrial Function and Regulating the ERK Signaling Pathway

Figure 6

Curcumin pretreatments alleviated mitochondria damage in oxygen-glucose deprivation/reoxygenation (OGD/R)-injured astrocyte. (a) Flow cytometry detection of alterations in JC-1 (fluorescence color reflecting alterations in the mitochondrial membrane potential). (b) Summary of data of the alterations of mitochondrial membrane potential. (c) Summary of data showing levels of intracellular adenosine triphosphate (ATP). (d–g) Representative images and summarized data for relative protein expressions of phosphorylated dynamin-related protein 1 (p-DRP1; Ser 637), DRP1, and mitofusin 2 (MFN2) by western blot analysis. (h) Representative images of transmission electron microscopy of astrocytic mitochondria. Versus control group, , , , respectively; versus OGD/R group, ^#, ^##, and ^### (n = 3).

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