Research Article
Icariin Regulates the hsa_circ_0003159/eIF4A3/bcl-2 Axis to Promote Gastric Cancer Cell Apoptosis
Figure 1
Validation of interaction between hsa_circ_0003159 and eIF4A3 or eIF4A3 and Bcl-2. ((a)–(c)) Western blotting showed the elF4A3 expression in the cytoplasm and nucleus in GC cell lines transfected with circ_0003159 overexpression plasmid (circ_0003159) or empty plasmid (Vector), and the ratio was quantified. (d) RNA immunoprecipitation (RIP) of elF4A3 in GC cell lines that transfected with circ_0003159 or Vector. Circ_0003159 or GAPDH bound to elF4A3 were determined using qRT-PCR. Input of circ_0003159 was normalized to the Vector group. (e) RNA Pull down experimental result indicated the interaction between hsa_circ_0003159 and eIF4A3. (f) Using anti-eIF4A3, RIP experimental result showed the enrichment of bcl-2 in the lysates from GC cell. (g) BGC-803 and (h) HGC-27 cell were transfected with si-eIF4A3 followed by Actinomycin D treatment, and the bcl-2 mRNA expression was quantified by qRT-PCR. vs. Vector group or siRNA group.
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