Icariin Regulates the hsa_circ_0003159/eIF4A3/bcl-2 Axis to Promote Gastric Cancer Cell Apoptosis

Figure 2

Hsa_circ_0003159 inhibit bcl-2 expression by recruiting eIF4A3 protein. (a) qRT-PCR detected the bcl-2 mRNA expression in GC cell transfected with circ_0003159 overexpression vector and/or si-eIF4A3. ((b)-(c)) Western blotting detected showed the bcl-2 protein expression in GC cell transfected with circ_0003159 overexpression vector and/or si-eIF4A3. (d) qRT-PCR detected the bcl-2 mRNA expression in GC cell transfected with circ_0003159 and/or eIF4A3 overexpression vector. ((e)-(f)) Western blotting detected showed the bcl-2 protein expression in GC cell transfected with circ_0003159 and/or eIF4A3 overexpression vector. (g) RIP of bcl-2 in GC cell after transfection with circ_0003159 or Vector. Circ_0003159 and GAPDH bound to bcl-2 were determined using qRT-PCR. Input of circ_0003159 was normalized to the Vector group. (h) BGC-803 and (i) HGC-27 cell were transfected with circ_0003159 and/or eIF4A3 overexpression vector, followed by Actinomycin D treatment, and the bcl-2 mRNA expression was quantified by qRT-PCR. vs. Vector + siRNA/NC or Vector group. vs. circ_0003159 + si-eIF4A3/NC or circ_0003159 group.

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