Composition and Bioactivity of a Modified Huang-Lian-Jie-Du Decoction

Figure 1

The HPLC chromatogram of the mHLJD decoction. Column: Ascentis Express 90A C18 (4.6 mm × 25 cm; 5 µm; Supelco); mobile phase: 0.05% trifluoroacetic acid–acetonitrile–methanol (0 min, 90 : 5:5; 55 min, 50 : 5 : 45; 56 min, 0 : 0 : 100; 60 min, 0 : 0 : 100; 61 min, 90 : 5 : 5; and 65 min, 90 : 5 : 5); flow rate: 1.0 mL/min; recorded at 230 nm; column temperature maintained at 40°C. 1: gentiopicrin; 2: geniposide; 3: astilbin; 4: berberine chloride; and 5: baicalin.