Research Article
Composition and Bioactivity of a Modified Huang-Lian-Jie-Du Decoction
Figure 2
The chromatograms of seven reference markers: (a) gentiopicrin; (b) astilbin; (c) geniposide; (d) forsythoside A; (e) berberine chloride; (f) phillyrin; and (g) baicalin. Column: Ascentis Express 90A C18 (4.6 mm × 25 cm; 5 µm; Supelco); mobile phase: 0.05% trifluoroacetic acid–acetonitrile–methanol (0 min, 90 : 5 : 5; 55 min, 50 : 5 : 45; 56 min, 0 : 0 : 100; 60 min, 0 : 0 : 100; 61 min, 90 : 5 : 5; and 65 min, 90 : 5 : 5); flow rate: 1.0 mL/min; column temperature maintained at 40°C. Chromatograms were recorded at 230 nm for geniposide, berberine chloride, phillyrin, and forsythoside A, and 280 nm for gentipicrin, astilbin, and baicalin.
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