Composition and Bioactivity of a Modified Huang-Lian-Jie-Du Decoction

Figure 5

Effects of TNF-α and the mHLJD decoction extract on cytokine production in A431 cells. (a) The cytokine name of each dot on the Human Cytokine Array C5. POS: positive control spot and NEG: negative control spot. (b) Array results of culture medium from control, TNF-α, and mHLJD plus TNF-α groups. A431 cells were incubated with (TNF-α group and mHLJD extract plus TNF-α group) or without (control) 25 ng/mL TNF-α for 6 h. In the mHLJD extract plus TNF-α group, 0.5 mg/mL of the mHLJD decoction extract was added 1 h before TNF-α treatment. The culture medium was collected for blotting with the array membrane. Black frame: positive and negative control spot; blue frame: TNF-α; and red frame: spots of IL-1β (C2), IL-8 (J2), M-CSF (H3), and TGF-β2 (F8). (c) The normalized quantification values of 12 dots including 4 cytokines in 3 groups.

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