[Retracted] Cantharidin Inhibits Proliferation of Liver Cancer by Inducing DNA Damage via KDM4A-Dependent Histone H3K36 Demethylation

Figure 5

KDM4A silencing attenuates cantharidin-induced DNA damage in hepatoma cells. (a) KDM4A mRNA levels in the control and KDM4A silenced (siRNA) HepG2 were detected by qRT-PCR. (b) Western blot detection of KDM4A and H3K36me3 expression changes. KDM4Ai-HepG2 cells were treated with cantharidin (10 μM) for 48 h. (c) The expression of H2AX was detected after different treatments, and the DNA damage of liver cancer cells was analyzed. KDM4Ai-HepG2 cells were treated with cantharidin (10 μM) for 48 h. (d) The expression of p21 was detected after different treatments, and the DNA damage of liver cancer cells was analyzed. Cells were treated with cantharidin (10 μM) for 48 h. (e) CCK-8 detection of HepG2 cells with siRNA KDM4A. Cells were treated with cantharidin (10 μM) for 48 h. , .

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