Electroacupuncture Pretreatment Exhibits Lung Protective and Anti-Inflammation Effects in Lipopolysaccharide-Induced Acute Lung Injury via SIRT1-Dependent Pathways

Figure 4

EA pretreatment promoted SIRT1 expression and reduced the activation of NF-κB in lung tissues in LPS-induced ALI. (a) Immunohistochemistry analysis for SIRT1 in mouse lung tissues. (40×) (b–d) Western blot and Rt-PCR analysis for SIRT1 expression. Western blot analysis for NF-κB (e, f) and ac–NF–κB (g, h) expression. The data are presented as the mean ± SEM. versus control group and ^# and ^### versus LPS group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)