Evidence-Based Complementary and Alternative Medicine

Research Article

Mechanical Study of Jian-Gan-Xiao-Zhi Decoction on Nonalcoholic Fatty Liver Disease Based on Integrated Network Pharmacology and Untargeted Metabolomics

Table 1

The differential metabolites in the serum after JGXZ treatment.


m/z	Rt (min)	Formula	Metabolites	VIP		FC		Trend		Pathway
m/z	Rt (min)	Formula	Metabolites	M vs. C	J vs. M	M vs. C	J vs. M	M vs. C	J vs. M	Pathway

116.07	1.06	C₅H₉NO₂	L-Proline	2.43	1.07	4.47	0.49	↑^##	↓	h
120.07	0.92	C₄H₉NO₃	L-Threonine	1.18	1.29	0.27	2.30	↓	↑	—
147.11	0.76	C₆H₁₄N₂O₂	L-Lysine	1.38	0.45	2.14	0.99	↑^##	↓	—
146.12	1.05	C₇H₁₅NO₂	Acetylcholine	1.18	0.43	0.68	1.07	↓^#	↑	e
198.08	0.96	C₆H₁₃N₃O₃	Citrulline	1.47	0.89	3.13	0.77	↑^##	↓	d
203.08	5.77	C₁₁H₁₂N₂O₂	L-Tryptophan	1.52	2.02	2.62	0.59	↑^##	↓	b
132.10	2.69	C₆H₁₃NO₂	L-Isoleucine	2.94	0.55	2.19	0.86	↑^##	↓	—
118.09	1.43	C₅H₁₁NO₂	L-Valine	2.79	0.66	2.72	0.85	↑^##	↓	—
281.25	0.71	C₁₈H₃₂O₂	Linoleic acid	1.28	1.02	0.76	2.05	↓^##	↑	a
175.12	0.88	C₆H₁₄N₄O₂	L-Arginine	2.77	1.45	1.87	0.48	↑^#	↓	d, h
380.25	9.54	C₁₈H₃₈NO₅P	Sphingosine-1-phosphate	2.17	1.60	1.97	0.51	↑^##	↓	—
132.10	2.92	C₆H₁₃NO₂	L-Leucine	3.89	1.74	1.77	0.68	↑^#	↓	—
301.22	10.42	C₂₀H₃₀O₂	Eicosapentaenoic acid	1.06	1.79	0.31	2.86	↓^##	↑	—
464.30	8.80	C₂₆H₄₃NO₆	Glycocholic acid	1.73	1.78	2.31	0.25	↑^#	↓	—
169.03	2.30	C₅H₄N₄O₃	Uric acid	2.37	0.09	3.31	0.90	↑^##	↓	—
283.26	14.23	C₁₈H₃₆O₂	Stearic acid	2.30	2.07	1.72	0.61	↑^#	↓	—
216.06	6.60	C₅H₁₄NO₆P	Glyceryl phosphoryl ethanolamine	1.22	1.96	2.58	0.49	↑^#	↓	e
255.23	11.44	C₁₆H₃₂O₂	Palmitic acid	2.09	0.61	2.83	0.84	↑^##	↓	—
277.22	10.50	C₁₈H₃₀O₂	Alpha-linolenic acid	1.60	1.98	0.44	2.11	↓^##	↑	c
241.06	9.83	C₆H₁₂O₇	Gluconic acid	1.23	0.43	0.63	1.08	↓^##	↑	—
948.80	0.84	C₆₁H₁₀₂O₆	TG(18 : 0/20 : 4(5Z,8Z,11Z,14Z)/20 : 4(5Z,8Z,11Z,14Z))	1.96	0.59	2.24	0.95	↑^##	↓	f
104.11	10.17	C₅H₁₃NO	Choline	2.19	1.84	0.70	1.76	↓^#	↑	e
766.48	10.58	C₄₅H₇₀NO₈P	Phosphatidylcholine	1.33	0.56	0.36	1.02	↓^##	↑	e
738.55	9.33	C₄₂H₈₀NO₈P	Phosphatidylethanolamine	1.13	1.08	2.74	0.46	↑^##	↓	a, c, e
115.04	1.06	C₃H₈O₃	Glycerol	0.25	0.04	2.79	0.98	↑^##	↓	f
319.23	9.43	C₂₀H₃₂O₃	12(R)-HETE	0.68	1.90	2.44	0.56	↑^##	↓	—
181.07	10.14	C₆H₁₂O₆	D-Galactose	0.16	1.14	1.30	0.71	↑^##	↓

Control, model, and JGXZ (n = 10 per group) groups. ^# as compared to the control group; ^## as compared to the control group; as compared to the model group; as compared to the model group; ↑: content increased; ↓: content decreased; vs.: versus; C: control group; M:model group; J:JGXZ group; Rt: retention time; VIP: variable importance of projection; FC: fold change a: linoleic acid metabolism; b: tryptophan metabolism; c: alpha-linolenic acid metabolism; d: arginine biosynthesis; e: glycerophospholipid metabolism; f: glycerolipid metabolism; : galactose metabolism; h: arginine and proline metabolism.