Sweet Wormwood and Tortoise Shell Decoction (Thanh Hao Miet Giap Thang) Induces DNA Damage, S-Phase Arrest, and Apoptosis in MCF-7 Cells via ATR-CHK1 Signaling Pathway

Figure 3

DSB accumulation in MCF-7 cells treated with THMGT. (a) Immunofluorescence staining of γ-H2AX foci in MCF-7 cells and ph-fibroblasts. Cells were treated with THMGT at a concentration of 3 mg/ml for 3 h and stained with a primary antibody against γ-H2AX and FITC-conjugated secondary antibody. Nuclei were counterstained with Hoechst. The data presented are representatives of three independent experiments. (b) Quantitation of the immunofluorescence staining data obtained from three independent experiments is shown . (c) Antiproliferative effects of THMGT on MCF-7 cells and ph-fibroblast. Cells were treated with THMGT at a concentration of 3 mg/ml for 3, 6 and 9 h and the proliferation was determined by the MTT assay. Data are shown as mean ± SD from at least three independent experiments.

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