[Retracted] Role of miR-584-5p in Lipopolysaccharide-Stimulated Human Bronchial Epithelial Cell Inflammation and Apoptosis

Figure 4

Effect of miR-584-5p on apoptosis of lipopolysaccharide (LPS)-stimulated 16-HBE cells. 16-HBE cells were divided into eight groups: mimic negative control (mimicNC), mimic miR-584-5p (mimic584), mimic negative control + LPS (mimicNC + LPS), miR-584-5p mimic + LPS (mimic584 + LPS), inhibitor negative control (inhibitorNC), inhibitor miR-584-5p (inhibitor584), inhibitor negative control + LPS (inhibitorNC + LPS), and inhibitor miR-584-5p + LPS (inhibitor584 + LPS). 16-HBE cells in the mimicNC and mimicNC + LPS groups were transfected with mimic negative control (mimicNC); mimic584 and mimic584 + LPS groups were transfected with mimic miR-584-5p (mimic584); inhibitorNC and inhibitorNC + LPS groups were transfected with inhibitor negative control (inhibitorNC); and inhibitor584 and inhibitor584 + LPS groups were transfected with inhibitor miR-584-5p (inhibitor584). Apoptosis in the mimicNC-, mimic584-, inhibitorNC-, and inhibitor584-transfected 16-HBE cells after LPS treatment was evaluated using western blotting. The protein expression of GAPDH, Bax, Bcl-2, cleaved caspase 3, and cleaved caspase 12 in all groups was measured using western blotting. p < 0.05 compared with the mimicNC, mimic584, and mimicNC + LPS groups; #p < 0.05 compared with the mimic584 and mimicNC + LPS groups; △p < 0.05 compared with the mimicNC, mimicNC + LPS, and mimic584 + LPS groups. p < 0.05 compared with the inhibitor584, inhibitorNC + LPS, and inhibitor584 + LPS groups; ^#p < 0.05 compared with the inhibitorNC group; △ p < 0.05 compared with the inhibitorNC and inhibitor584 groups; ^▲p < 0.05 compared with the inhibitorNC and inhibitorNC + LPS groups. The experiments were performed at least three times independently. Data are presented as mean ± standard deviation (SD).