A Study of the Protective Effect of Bushen Huoxue Prescription on Cerebral Microvascular Endothelia Based on Proteomics and Bioinformatics

Figure 4

BSHX prevented FoxO1 and FoxO3 proteins from translocating into the nucleus and promoted their degradation under AGE-induced stimulation. (a) bEnd.3 cells were treated with AGEs (300 μg/mL) with or without BSHX (20, 50, and 100 mg/mL, respectively) for 72 h. The total expression of FoxO1 and FoxO3 was determined by Western blot assay. (b) The nuclear and cytoplasmic expression of FoxO1 and FoxO3 in bEnd.3 cells treated with AGEs (300 μg/mL) with or without BSHX (100 mg/mL) for 1 h was determined by Western blot assay. Histone H3 and α-tubulin were utilized as internal controls for nuclear and cytoplasmic proteins, respectively. (c) bEnd.3 cells were treated as in (a) for 72 h and then the expression of Bim and cleaved caspase-3 was detected by Western blot assay. All data were acquired by at least three independent experiments and presented as mean ± S.D. , relative to the control group; , relative to the model group.

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