The Clinical Effect and Mechanism of Prostant on Urinary Retention and Anal Pain

Figure 3

Prostant regulated inflammatory factors expression through modulating Th17/Treg balance with inhibiting JAK/STAT axis. (a) The serum level of hs-CRP, TNF-α, IL-2, and IL-6 was examined via ELISA. ((b) and (c)) The level of VEGF in mucosal tissues was determined by IHC. ((d) and (e)) The expression of Th17 and Treg was assessed by flow cytometry assay. ((f) and (g)) The protein expression of STAT3, phosphorylated STAT3, SOCS3, and JAK2 was evaluated by western blot. Data were exhibited following being normalized to GAPDH. The means ± SD of six independent samples were displayed. and v.s control group. , and v.s H&UR model group.

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