Exploring the Mechanism of Astragalus propinquus Schischkin and Panax Notoginseng (A&P) Compounds in the Treatment of Renal Fibrosis and Chronic Kidney Disease Based on Integrated Network Analysis

Figure 1

Flowchart of investigation on the mechanism of A&P in the treatment of renal fibrosis in chronic kidney disease. First, the potential active components and targets were obtained from TCMSP and Target Prediction database. Second, CKD-related microarrays on NCBI were used to obtain disease-related targets. The intersection of active ingredient targets and disease targets is regarded as a common target. Thirdly, Go and KEGG enrichment analyses were performed for common targets. The differences of common targets were analyzed and visualized; heatmap was made. Cytoscape is used to construct the regulatory network of TCM. Then, UUO model was constructed to verify the hub gene and determine the effects of A&P. PyMOL is used for molecular docking of potentially active components and hub genes.